From synthesis to bioactivity: A comprehensive study of Cu-based biocidal tool

Abstract

The present study reports on the biogenic synthesis of a copper-based biocidal material through the fermentation of gruel, a traditional non-alcoholic beverage. This process may involve a bio-beneficiation mechanism, in which the indigenous microorganisms in the ferment interact with the material. X-ray diffraction analysis confirmed the powder's crystalline copper composition. Fourier-transform infrared spectroscopy revealed the crucial role of organic acids in the capping process. Transmission electron microscopy, ultraviolet-visible spectroscopy, and antimicrobial susceptibility tests were conducted to characterize the powder. Furthermore, the biocidal material was combined with the anticancer drug curcumin to explore its additional anti-proliferative effects, including apoptosis, on human hepatocellular carcinoma cell lines in vitro. These findings highlight the potential of this biogenic copper material as a promising candidate for biomedical applications.