Dynamic Protective Potential of Couroupita guianensis. Aubl against Reactive Free Radicals: An Approach of Stimulated in vitro model.

Abstract

Oxygen is an essential element for life but it is also the reason for the cause of tissue damage. During various metabolic activities, oxygen gets reduced to highly reactive oxygen metabolite which readily reacts with macromolecules like protein, carbohydrate, lipid and nucleic acids in the body. Antioxidants block the process of oxidation by neutralizing these reactive free radicals. The present study has focused on the validation of the antioxidant potential of Couroupita guianensis.Aubl. using goat liver slices which is an in vivo stimulated in vitro model system. Liver is the major metabolic organ involved in clearance of many xenobiotics. Hydrogen peroxide was used as the standard oxidant in treatment groups. The enzymatic and non enzymatic antioxidant potential was evoked by the methanolic extract and alkaloid fraction of both leaves and flowers of Couroupita guianensis.Aubl. in oxidatively stressed goat liver slices. The results proved that the Couroupita guianensis.Aubl. exhibits good antioxidant potential and is able to combat oxidative damaging systems for preventing various oxidative stress induced secondary diseases and disorders. This in vitro model system will mitigate the sacrifice of animals by mimicking the in vivo condition for research purposes.