GCMS analysis & assessment of antimicrobial potential of rhizospheric Actinomycetes of AIA3 isolate

Abstract

Plants have been used for medicine to support human health in many regions in the world by researchers since ancient times. Plants and soil organisms have been found to have very high therapeutic potential as they produce many natural products. Evolving drug resistance towards nearly all anti-infection drugs, lead to the fast development of new drugs. Many natural products or secondary metabolites have been used for animal and human health. Recently, many new secondary metabolites from actinomycetes have been isolated and reported as important compounds with different activities like
anti-microbial, anti-oxidant, anti-inflammatory, anti-androgenic and anticancer agents, etc. In this study isolation of actinomycetes was carried out on actinomycetes isolation agar media (AIA). Characterization and biochemical tests were performed and followed by fermentation and solvent extraction by four solvents for example- Benzene, pet ether, ethyl acetate, chloroform. GCMS was performed for identification of compounds present in culture broth. Major compounds present were Octanal,Pyrrolo[1,2-a]pyrazine-1,4-dione,hexahydro-3-(2-methylpropyl), Dibutyl phthalate, N-hexadecanoic acid, 1-nonadecene, Heptadecane, Octadecanoic acid, 3,7,11,15-tetramethyl-2-hexadecene, Dihydroergotamine, Hexadecanoic acid, 2-hydroxy-1-(hydroxymethyl) ethyl ester, Octadecanoic acid, 2,3-dihydroxypropyl ester, 13-docosenamide, and 4-tert-butylcalix[4]arene. Crude obtained was checked for their antimicrobial activity and inhibition zones (IZ) were noted on Mullar Hinton agar (MHA) media against indicator organisms like Staphylococcus aureus (MTCC-3160) (IZ=Ben-18 mm, E.A-25 mm), Pseudomonas aeruginosa (MTCC 1688) (IZ=Ben-11 mm, Chl-14 mm, E.A-24 mm), Klebsiella pneumonia (MTCC-432) (IZ=Ben-19 mm, Chl-20 mm, E.A-34 mm), Proteus vulgaris (MTCC-7306) (IZ=Benzene-10 mm, E.A-30 mm), Bacillus subtilis (MTCC-441). Identification of compounds was carried out by NIST 14 library.